Taking Cuttings & The PermaClone Recipe

Cloning Cuttings & ”The PermaClone Recipe”

Our last article provided a to-do and shopping list (did you complete it? I hope you did!).

First, I asked that you foliar spray your mother plants with OMRI Certified Pesticide (Such as Garden Safe Neem or take-down) containing a Kelp-based extract 24-48 hours before taking cuts.

I also outlined three ingredients required for reliable cloning: Oxidizer, Water Soluble Hormone, mineral nutrients, and Fiskars.

Your mother plants should have natural auxins and amino acids coursing through their stems and your cloning station should be stocked with three cloner additives and a pair of fiskars.

Today it’s time to clone!

Fill up your sterilized cloner with water and immediately add a plant safe dose of your oxidizer of choice. Here’s the dosages:

Product

Dosage

Sodium Hypochlorite (e.g. bleach)

0.1 - 0.15 mL/gal (2 - 3 drops/gal)

Hypochlorous Acid (e.g. UC Roots)

10 mL/gal

30% Peroxide (e.g. Nutrilife)

3 mL/gal

Peroxyacetic acid (e.g. Zerotol)

2 mL/gal

 

Take "clones" or cuttings 

More Nodes & Shoot Apices: Every point along the stems where a new shoot and fan leaf forms is called a “node”. The tip of each shoot from this node is called the shoot apex or terminal bud. Each shoot apex produces auxins (rooting hormones). When taking your cuttings, the goal is to leave several nodes above the cloning collar (untrimmed) from your cutting because each shoot apex manufactures natural auxins that travel to the bottom (basal) end of your aeroponic cutting or clone. When taking your cutting, the goal is 3 – 6 nodes left untrimmed which will go above the collars and 2 - 3 node trimmed which will go below the collar. This requires that when you take cutting from your mother plant, choosing segments that are 5 - 9 nodes tall. Before inserting them in a sterilized collar, trim off the lower 2 - 3 nodes (including the leaves) and leave the top 3 - 6 nodes untrimmed.

Removing Leaves: Removing fan leaves was a common practice before water culture cloning. Now we only recommend removing fan leaves to avoid shading other clones. The tradition of removing fan leaves was to decrease water loss through the pores in the leaves (stomata). In water-culture your clones' stems are well hydrated such that this water loss (transpiration) is not a concern. We only  recommend trimming leaves to avoid shading other clones. If you're aiming for 3 - 4 cuts (or clones) per collar, eliminate ALL fan leaves and leave JUST nodes and shoot apices. And, YES...it works. This method alone with make your cloner 3 - 4x more productive! 

Keep Track of Strains: As you Add cuttings to the system, keeping track of your strains by rows on paper. I recommend taking a picture of that paper so you have a digital backup for transplant day in your smart phone.

By the time all your aeroponic clones or cuttings are in place your initial boost of sterilizer has depleted. It’s important that before adding nutrients you boost your sterilizer a second time as listed below to surface sterilize everything before additives. This IS VERY IMPORTANT! ...add your oxidizer of choice before additive because most additive deplete the sterilizers: 

Product

Dosage

Sodium Hypochlorite (e.g. bleach)

0.1 - 0.15 mL/gal (2 - 3 drops/gal)

Hypochlorous Acid (e.g. UC Roots)

10 mL/gal

30% Peroxide (e.g. Nutrilife)

2 mL/gal

Peroxyacetic acid (e.g. Zerotol)

2 mL/gal

 

Next, add your hormone solution (only use one of the 3 recommended):

  • Hormex Liquid Concentrate: 3 - 5 mL/gal
  • KLN Cloning Solution: 5 - 15 mL/gal
  • Rhizopon AA Salts: 0.15 g/gal - 0.4 g/gal 

 

Last add your mineral Solution: 

  • Clonex Solution: 20 - 30 mL/gal
  • Cutting Edge Solution: 2.5 mL/gal Micro, 5 mL/gal Bloom
  • General Hydroponic: 2.5 mL/gal Micro , 5 mL/gal Bloom

Do not pH adjust your system until roots have formed. pH adjustment risks infecting your system with unwanted microbes and is tedious. Remember, we're all about simple work-flow.  

Set your cycle timer to 5 - 45 min on and 5 - 30 min off. Remember to adjust your on/off time to achieve and external IR temperature of 75 - 87 F.

**Use your IR thermometer to maintain/monitor temperatures.

Other than daily IR temperature checks, leave your system alone for 7 days. Eventually you’ll have you Cycle timing dialed in and won’t have to check temperatures as frequently. between days 3  - 5 you should have callous tissue (which, cancerous-looking bumps/growths) on the stem inside the system. at 5 - 7 days roots should be formed if you hit all the targets in this series. 


Keep in touch,


Michael Goldsmith

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PS: My articles share knowledge I've accumulated over the years while perfecting my use of PermaClone collars and collaborating with 1000's of customers. My hope is by sharing this knowledge, PermaClone's plant collars will be acknowledged as the best cloning pucks on the market for hydroponic, deep-water culture, and aeroponic systems. Please spread the word! PermaClone has collar sizes to fit the top cloning systems: EZ-CLONE, TurboKlone, PowerCloner by Botanicare, Oxyclone, Psychloner and more. We can also recommended sizes for DIY and home-made cloning systems...just reach out for dimension. Thanks for your support! #PermaClone #getsterilegetcloning 


11 comments

  • Great knowledge. Thanks

    Freshwaterjellyfish
  • Let’s say I’m running an ebb and flow and using uc roots but I’m also running silica such as protekt from dyna gro. Do you think I should add the uc roots to the water first or the protekt? I’ve always been told to use silica first. Thanks!

    Dom
  • Can I use GH Rapid Start instaed of hormex, KLN Cloning Solution or Rhizopon AA Salts??
    If yes how many ml per gallon?

    gerard
  • Just wondering what your thoughts are about misting clones in the cloner once they are set. Many write-ups suggest misting once or twice a week. I’m guessing that since the bubbler is on, the dome gets plenty of high humidity and fresh air so there is no need to risk possible contaminating the somewhat sterile environment…..and also, there is no need to open the vents since air is injected via the air stones. Anyway, am I interpreting that right even though you didn’t explicitly say so?

    Richard Scorzelli
  • Every time I’ve tried using a bubble cloner in the past has been a failure. I gave away the last one I had. Recently I found a pump on clearance at Lowe’s and decided to try it again and it’s went very well. The difference is that this time I ran across your site. Since I started using the method outlined in this post my success rate has been in the high 90s, and I’ve hit 100% a few times.

    Thank you very much for sharing this.

    Cliff

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